Scar

Part:BBa_K2355317:Design

Designed by: Chrysillis Polhaus   Group: iGEM17_DTU-Denmark   (2017-10-26)


Double cut side containing NgoMIV and AgeI, with a frameshift and stop codon in between.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1
    Illegal AgeI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 12


Design Notes

Scar for putting in linkers...


Source

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References